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MdERDL6-mediated glucose efflux to the cytosol promotes sugar accumulation in the vacuole through up-regulating TSTs in apple and tomato

Proc Natl Acad Sci U S A. 2020-12; 
Lingcheng Zhu, Baiyun Li, Limin Wu, Huixia Li, Zhengyang Wang, Xiaoyu Wei, Baiquan Ma, Yanfeng Zhang, Fengwang Ma, Yong-Ling Ruan, Mingjun Li
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摘要

Sugar transport across tonoplasts is essential for maintaining cellular sugar homeostasis and metabolic balance in plant cells. It remains unclear, however, how this process is regulated among different classes of sugar transporters. Here, we identified a tonoplast H/glucose symporter, MdERDL6-1, from apples, which was highly expressed in fruits and exhibited expression patterns similar to those of the tonoplast H/sugar antiporters MdTST1 and MdTST2. Overexpression of unexpectedly increased not only glucose (Glc) concentration but also that of fructose (Fru) and sucrose (Suc) in transgenic apple and tomato leaves and fruits. RNA sequencing (RNA-seq) and expression analyses showed an up-regulation of and in t... More

关键词

H+/glucose symporter, H+/sugar antiporter, cytosolic sugar signaling, intracellular communication, sugar transport