至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Combined and Distinct Roles of Agr Proteins in Clostridioides difficile 630 Sporulation, Motility, and Toxin Production

MBio. 2020-12; 
Ummey Khalecha Bintha Ahmed, Tyler M Shadid, Jason L Larabee, Jimmy D Ballard
Products/Services Used Details Operation
PCR Cloning and Subcloning  inserted into the multiple-cloning site (MCS) of pMTL84151 (32) by GenScript Biotech to generate pTMS001 Get A Quote

摘要

The accessory gene regulator 1 () locus consists of two genes, and , that presumably constitute an autoinducing peptide (AIP) system. Typically, AIP systems function through the AgrB-mediated processing of AgrD to generate a processed form of the AIP that provides a concentration-dependent extracellular signal. Here, we show that the 630 Agr1 system has multiple functions, not all of which depend on AgrB1. CRISPR-Cas9n deletion of , a, or the entire locus resulted in changes in transcription of sporulation-related factors and an overall loss in spore formation. Sporulation was recovered in the mutants by providing supernatant from stationary-phase cultures of the parental strain. In contrast, motility was r... More

关键词

Agr, Cas9, Clostridioides difficile, Clostridium difficile, TcdA, TcdB, autoinducing peptide, sporulation