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Extracellular vesicles-encapsulated let-7i shed from bone mesenchymal stem cells suppress lung cancer via KDM3A/DCLK1/FXYD3 axis

J Cell Mol Med. 2020-12; 
Jiefeng Liu, Yuhua Feng, Xinyu Zeng, Miao He, Yujing Gong, Yiping Liu
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Mutant Libraries KDM3A 3’‐UTR sequences containing a mutant (MUT) or wild‐type (WT) let‐7i binding site was designed and constructed by GenScript (Nanjing, China)  Get A Quote

摘要

Accumulating evidence has suggested that extracellular vesicles (EVs) play a crucial role in lung cancer treatment. Thus, we aimed to investigate the modulatory role of bone marrow mesenchymal stem cell (BMSC)-EV-derived let-7i and their molecular mechanism in lung cancer progression. Microarray-based analysis was applied to predict lung cancer-related miRNAs and their downstream genes. RT-qPCR and Western blot analyses were conducted to determine Let-7i, lysine demethylase 3A (KDM3A), doublecortin-like kinase 1 (DCLK1) and FXYD domain-containing ion transport regulator 3 (FXYD3) expressions, after which dual-luciferase reporter gene assay and ChIP assay were used to identify the relationship among them. After ... More

关键词

FXYD domain-containing ion transport regulator 3, bone marrow mesenchymal stem cell, doublecortin-like kinase 1, extracellular vesicles, let-7i, lung cancer, lysine demethylase 3A