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Plasmid DNA Preparation> | … Plasmids. The Flot2-mEGFP plasmid was obtained by amplifying the flotillin 2 sequence (NM_004475.3; template: Flot_2_pcDNA/Hygro+ GenScript) in a PCR reaction (forward primer … | Get A Quote |
MPP1 (membrane palmitoylated protein 1) belongs to the MAGUK (membrane-associated guanylate kinase homologs) scaffolding protein family. These proteins organize molecules into complexes, thereby maintaining the structural heterogeneity of the plasma membrane (PM). Our previous results indicated that direct, high-affinity interactions between MPP1 and flotillins (raft marker proteins) display dominant PM-modulating capacity in erythroid cells. In this study, with high-resolution structured illuminated imaging, we investigated how these complexes are organized within erythroid cells on the nanometer scale. Furthermore, using other spectroscopic techniques, namely fluorescence recovery after photobleaching (FRAP) ... More