至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules

Cells. 2022-01; 
Agnieszka Biernatowska, Karolina Wójtowicz, Tomasz Trombik, Aleksander F Sikorski, Aleksander Czogalla
Products/Services Used Details Operation
Plasmid DNA Preparation … Plasmids. The Flot2-mEGFP plasmid was obtained by amplifying the flotillin 2 sequence (NM_004475.3; template: Flot_2_pcDNA/Hygro+ GenScript) in a PCR reaction (forward primer … Get A Quote

摘要

MPP1 (membrane palmitoylated protein 1) belongs to the MAGUK (membrane-associated guanylate kinase homologs) scaffolding protein family. These proteins organize molecules into complexes, thereby maintaining the structural heterogeneity of the plasma membrane (PM). Our previous results indicated that direct, high-affinity interactions between MPP1 and flotillins (raft marker proteins) display dominant PM-modulating capacity in erythroid cells. In this study, with high-resolution structured illuminated imaging, we investigated how these complexes are organized within erythroid cells on the nanometer scale. Furthermore, using other spectroscopic techniques, namely fluorescence recovery after photobleaching (FRAP) ... More

关键词

MPP1, flotillins, raft nanodomains