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The N-terminal helices of amphiphysin and endophilin have different capabilities of membrane remodeling

Biochim Biophys Acta Biomembr. 2022-03; 
Chinta M Aryal, Nhat Nguyen Bui, Likai Song, Jianjun Pan
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Stable Cell Lines … Louis, MO). Nickel(II) ethylenediaminediacetate (NiEDDA) was synthesized following the reported protocol [16]. H0-Amph was synthesized by GenScript … Get A Quote

摘要

Amphiphysin and endophilin are two members of the N-BAR protein family. We have reported membrane interactions of the helix 0 of endophilin (H0-Endo). Here we investigate membrane modulations caused by the helix 0 of amphiphysin (H0-Amph). Electron paramagnetic resonance (EPR) spectroscopy was used to explore membrane properties. H0-Amph was found to reduce lipid mobility, make the membrane interior more polar, and decrease lipid chain orientational order. The EPR data also showed that for anionic membranes, H0-Endo acted as a more potent modulator. For instance, at peptide-to-lipid (P/L) ratio of 1/20, the peak-to-peak splitting was increased by 0.27 G and 1.89 G by H0-Amph and H0-Endo, respectively. Simil... More

关键词

Amphipathic helix, Helix 0, Lipid bilayer structure, Lipid chain order, Membrane curvature generation, Membrane fluidity