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Synthesis and identification of lithocholic acid 3-sulfate as RORγt ligand to inhibit Th17 cell differentiation

J Leukoc Biol. 2022-02; 
Riping Xiao, Kawai Lei, Hioha Kuok, Wende Deng, Yuxin Zhuang, Yanqing Tang, Zhengyang Guo, Hongyan Qin, Li-Ping Bai, Ting Li
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Custom Vector Construction … RORγt ligand-binding domain (LBD) gene from human (Genbank acces- sion number NP_005051, aa 259–518) was constructed by the com- pany Genscript and cloned into pET21b … Get A Quote

摘要

Primary bile acids (BAs), products of cholesterol metabolism and clearance, are synthesized in the liver and released into the intestine to facilitate the digestion and absorption of lipids. BAs are further converted by gut commensal bacteria into secondary colonic BAs and the metabolism disorder is closely linked to cholestatic liver diseases via regulating immune response. However, the effect and underlying mechanism of these host-microorganism biliary metabolites on T lymphocyte remain unclear. In the current study, we synthesized a sulfated product of lithocholic acid (LCA), lithocholic acid 3-sulfate (LCA-3-S), and investigated the binding affinity of the BAs metabolites on RORγt, the transcription facto... More

关键词

RORγt, Th17 cells, lithocholic 3-sulfate