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Custom DNA/RNA Oligos> | … Mice were immunized sc on day 0 with 100 μg of myelin oligodendrocyte glycoprotein (MOG)35−55 peptide (N-MEVGWYRSPFSRVVHLYRNGK-C, GenScript) emulsified in CFA (Difco … | Get A Quote |
Activation of the Ca release-activated Ca (CRAC) channel is crucial for T cell functions. It was recently shown that naked cuticle homolog 2 (NKD2), a signaling adaptor molecule, orchestrates trafficking of ORAI1, a pore subunit of the CRAC channels, to the plasma membrane for sustained activation of the CRAC channels. However, the physiological role of sustained Ca entry via ORAI1 trafficking remains poorly understood. Using NKD2 as a molecular handle, we show that ORAI1 trafficking is crucial for sustained Ca entry and cytokine production, especially in inflammatory Th1 and Th17 cells. We find that murine T cells cultured under pathogenic Th17-polarizing conditions have higher Ca levels that are NKD2-dependen... More