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ORF cDNA Clones/MolecularCloud> | … 2.3. Overexpression of IL21R. Overexpression was performed using the GenEZ ORF clone of IL21R (NM_021798) con- structed with pcDNA3.1+/C-(K)-DYK vector (GenScript) … | Get A Quote |
Dysfunctions in adipose tissue cells are responsible for several obesity-related metabolic diseases. Understanding the process of adipocyte formation is thus fundamental for understanding these diseases. The adipocyte differentiation of adipose-derived stem/stromal cells (ADSCs) showed a reduction in the mRNA level of the interleukin 21 receptor (IL21R) during this process. Although the receptor has been associated with metabolic diseases, few studies have examined its function in stem cells. In this study, we used confocal immunofluorescence assays to determine that IL21R colocalizes with mitochondrial protein ATP5B, ALDH4A1, and the nucleus of human ADSCs. We demonstrated that silencing and overexpression of ... More