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Improved Performance of ELISA and Immunochromatographic Tests Using a New Chimeric A2-Based Protein for Human Visceral Leishmaniasis Diagnosis

J Immunol Res. 2021-04; 
Maria Marta Figueiredo, Anna R R Dos Santos, Lara C Godoi, Natália S de Castro, Bruno C de Andrade, Sarah A R Sergio, Selma M B Jerônimo, Edward J de Oliveira, Ruth T Valencia-Portillo, Lucilândia M Bezerra, Hiro Goto, Maria C A Sanchez, Caroline Junqueira, Santuza M R Teixeira, Flávio G da Fonseca, Ricardo T Gazzinelli, Ana Paula Fernandes
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Bacterial Expression … 3 Journal of Immunology Research Page 4. the E. coli expression vector pET24a was purchased from GenScript (Piscataway, NJ, EUA). E. coli BL21 … Get A Quote

摘要

unassigned: A total of 1028 sera samples were used for the development and validation of ELISA (321 samples from -infected patients, 62 samples from VL/AIDS coinfected patients, 236 samples from patients infected with other diseases, and 409 samples from healthy donors). A total of 520 sera samples were used to develop and validate ICT (249 samples from -infected patients, 46 samples from VL/AIDS coinfected patients, 40 samples from patients infected with other diseases, and 185 samples from healthy donors). . Using the validation sera panels, DTL-4-based ELISA displayed an overall sensitivity of 94.61% (95% CI: 89.94-97.28), a specificity of 99.41% (95% CI: 96.39-99.99), and an accuracy of 97.02% (95% CI: 94.6... More

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