Products/Services Used | Details | Operation |
---|---|---|
Plasmid DNA Preparation> | … affinity purification. The recombinant plasmids pET20b-Ah-f and pET20b-CDh-f were synthesized by GenScript Biotech (Nanjing, China). The plasmid … | Get A Quote |
Various vaccine strategies have been developed to provide broad protection against diverse influenza viruses. The hemagglutinin (HA) stem is the major potential target of these vaccines. Enhancing immunogenicity and eliciting cross-protective immune responses are critical for HA stem-based vaccine designs. In this study, the A helix (Ah) and CD helix (CDh) from the HA stem were fused with ferritin, individually, or in tandem, yielding Ah-f, CDh-f and (A + CD)h-f nanoparticles (NPs), respectively. These NPs were produced through a prokaryotic expression system. After three immunizations with AS03-adjuvanted NPs in BALB/c mice the subcutaneous route, CDh-f and (A + CD)h-f induced robust humoral and cellular immu... More