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Developing and characterizing a single-domain antibody (nanobody) against human cytotoxic T-lymphocyte-associated protein 4 (hCTLA-4)

Iran J Basic Med Sci. 2021-09; 
Nazli Sotoudeh, Zahra Noormohammadi, Mahdi Habibi-Anbouhi, Fatemeh Kazemi-Lomedasht, Mahdi Behdani
Products/Services Used Details Operation
DNA Sequencing … sequencing results were analyzed in National Center for Biotechnology Information-Basic … of Nb 3hCTL55 on numerous proteins; human PD1 (Genscript Biotech, USA), recombinant … Get A Quote

摘要

unassigned: Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is the most important human immune checkpoint that modulates T cells activity and brings about immune-homeostasis. Accordingly, checkpoint inhibitor cancer therapy has been approved as a growing method to block over-expressed immune checkpoints, such as CTLA-4 receptors. Considering the competitive characteristics of single-domain antibodies with monoclonal antibodies, we tried to develop a camelid Nanobody against human CTLA-4. unassigned: We have constructed the VHH gene library by using immunized-camel peripheral blood mononuclear cells and carrying out the Nested-PCR technique. VHH-library was screened by phage display technique and specific n... More

关键词

CTLA-4 antigen, Immune checkpoint- proteins, Immunotherapy, Nanobody, Single-domain antibodies