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Increased sensitivity of gp210 autoantibody detection using a newly designed gp210 antigen

J Immunol Methods. 2021-12; 
Jing Xu, Haoyi Zhang, Chan Wang, Peng Jiang, Chongxu Han, Yaping Dai, Fang Qiu, Yuhua Gong, Yuzhang Jiang, Ping Xu, Mingming Zhang, Luyao Zhang, Xingjuan Shi, Sufang Chen, Ye Tian, Michael F Seldin, M Eric Gershwin, Xiangdong Liu, Li Li
Products/Services Used Details Operation
Mammalian Expression … diluted horseradish peroxidase-conjugated antihuman immunoglobulin G antibody (Genscript, China), the … The QUANTA Lite gp210 ELISA kit (INOVA Diagnostics, Inc), which uses a … Get A Quote

摘要

objectives: The detection of autoantibody to glycoprotein 210 (gp210 Ab) against a 15 amino-acid peptide epitope by enzyme-linked immunosorbent assay (ELISA) has been widely used in the diagnosis of primary biliary cholangitis (PBC). However, this small peptide antigen presents spatial limitations for antibody access, which reduces the sensitivity of autoantibody detection. A recombinant gp210 antigen was constructed for increased sensitivity in antibody detection is described here. methods: The gp210 C terminal 18 amino acid coding sequence was ligated to the modified C-terminal 108 amino acid coding sequence of human serum albumin (mHSA108) and produced as a recombinant gp210 antigen mHSA108-gp210-C18. Measur... More

关键词

Enzyme-linked immunosorbent assay, Glycoprotein 210, Human serum albumin, Primary biliary cholangitis