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Overexpression of sonic hedgehog enhances the osteogenesis in rat ectomesenchymal stem cells

Cell Tissue Bank. 2022-02; 
Weijiang Wu, Zhe Wang, Zhijian Zhang, Wenjing Yang, Xin Fan, Jili Xu, Zhiqiang Huang, Qixiang Shao
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Plasmid DNA Preparation … 5'-CGCGGATCC TCAGCTGGACTTGACTGCCATT-3'. A recombinant rShh adenovirus plasmid was constructed by Nanjing Genscript Bioengineering Technology and Services Co., Ltd … Get A Quote

摘要

Ectoderm-derived mesenchymal stem cells (EMSCs) were used as potential seed cells for bone tissue engineering to treat bone defects due to their capability of rapid proliferation and osteogenic differentiation. Sonic hedgehog (Shh) signaling was reported to play an important role in the development of bone tissue, but its role is not understood. The present study investigated the role of Shh molecule in osteogenic differentiation of rat EMSCs in vitro. Rat EMSCs were isolated form nasal respiratory mucosa and identified with immunofluorescence and analyzed with other methods, including reverse transcriptase polymerase chain reaction (qPCR) and western blotting. EMSCs expressed CD90, CD105, nestin, and vimentin.... More

关键词

Alkaline phosphatase, Ectomesenchymal stem cells, Gli1, Osteogenesis, Sonic hedgehog