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Plasmids for SARS-CoV-2 Detection and Research> | The expression plasmids used in this study were generated by GenScript from the parent plasmid (pNIC28-SmPutA),which encodes full-length SmPutA (UniProtKB F7X6I3, 1233residues) with an N-terminal His6 tag and TEVP cleavage site. | Get A Quote |
Proline dehydrogenase (PRODH) catalyzes the FAD-dependent oxidation of L-proline to Δ1-pyrroline-5-carboxylate and is a target for inhibitor discovery because of its importance in cancer cell metabolism. Because human PRODH is challenging to purify, the PRODH domains of the bacterial bifunctional enzyme proline utilization A (PutA) have been used for inhibitor development. These systems have limitations due to large polypeptide chain length, conformational flexibility, and the presence of domains unrelated to PRODH activity. Herein, we report the engineering of minimal PRODH domains for inhibitor discovery. The best designs contain about one-third of the 1233-residue parent PutA from Sinorhizobium meliloti and... More