Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | The pGEM®-T Easy vector (Promega Co., USA) and the pET-28a (+) vector (GenScript Co., USA) were used as the cloning and the expression vectors, respectively./The recombinant plasmid pET-28a (+)/AXE-HAS10 was constructed by GenScript Co., USA (Order: U3326EL100 _2) using BamH1 and Xho1 restriction enzymes. | Get A Quote |
Custom Vector Construction> | Get A Quote |
Acetylxylan esterase plays a crucial role in xylan hydrolysis as the acetyl side-groups restrict endoxylanase action by stearic hindrance. In this study, an acetylxylan esterase (AXE-HAS10: 960 bp & 319 a.a) putative ORF from Halalkalibacterium halodurans NAH-Egypt was extensively studied through heterologous overexpression in Escherichia coli, biochemical characterization, and structural modeling. The AXE-HAS10 tertiary structure was predicted by the Local Meta Threading Server. AXE-HAS10 belongs to the carbohydrate esterase Family 7. Purified to homogeneity AXE-HAS10 showed specific activity (36.99 U/mg), fold purification (11.42), and molecular mass (41.39 kDa). AXE-HAS10 showed optimal pH (8.5) and temperat... More