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Molecular study on recombinant cold-adapted, detergent- and alkali stable esterase (EstRag) from Lysinibacillus sp.: a member of family VI.

World J Microbiol Biotechnol .. 2022-09;

Amira A Matrawy, Ahmed I Khalil, Amira M Embaby

Products/Services Used	Details	Operation
Gene Synthesis	An open reading frame (ORF) (654 bp and 217 amino acids) from Lysinibacillus sp. encoding a novel cold-adapted esterase (EstRag) was artifcially synthesized by GenScript Biotech ® CO., USA./This ucleotide sequence encoding a novel cold adapted esterase (EstRag) was synthesized by GenScript Biotech®. Co., USA (U3326EL100 _4). /The full-length of esterase ORF (654 bp) from Lysinibacillus sp. YS11 reference sequence NZ_CP026007.1 of Lysinibacillus sp. YS11, with the protein ID: WP_103118438.1, was retrieved from GenBank and chemically synthesized by GenScript Co.	Get A Quote

摘要

Cold-adapted esterases have potential industrial applications. To fulfil the global continuous demand for these enzymes, a cold-adapted esterase member of family VI from Lysinibacillus sp. YS11 was cloned on pET-28b (+) vector and expressed in E. coli BL21(DE3) Rosetta cells for the first time. The open reading frame (654 bp: GenBank MT120818.1) encodes a polypeptide (designated EstRag: 217 amino acid residues). EstRag amino acid sequence has conserved esterase signature motifs: pentapeptide (GFSQG) and catalytic triad Ser110-Asp163-His194. EstRag 3D predicted model, built with LOMETS3 program, showed closest structural similarity to PDB 1AUO_A (esterase: Pseudomonas fluorescens); TM-align score program inferen... More