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Indolethylamine N-methyltransferase (INMT) is not essential for endogenous tryptamine-dependent methylation activity in rats

Scientific Reports. 2023-01; 
NicolasG.Glynos, Lily Carter, Soo Jung Lee, Youngsoo Kim , RobertT. Kennedy, GeorgeA. Mashour, Michael M. Wang & Jimo Borjigin
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Polyclonal Antibody Services Western blot analysis was performed with rat INMT (rabbit polyclonal custom ordered from GenScript), tubulin (mouse monoclonal from Developmental Studies Hybridoma Bank), and GST (mouse monoclonal from Fisher) antibodies, followed by incubation with infrared fuorophore-labeled secondary antibodies against rabbit and mouse (Li-COR). Get A Quote

摘要

Indolethylamine N-methyltransferase (INMT) is a transmethylation enzyme that utilizes the methyl donor S-adenosyl-L-methionine to transfer methyl groups to amino groups of small molecule acceptor compounds. INMT is best known for its role in the biosynthesis of N,N-Dimethyltryptamine (DMT), a psychedelic compound found in mammalian brain and other tissues. In mammals, biosynthesis of DMT is thought to occur via the double methylation of tryptamine, where INMT frst catalyzes the biosynthesis of N-methyltryptamine (NMT) and then DMT. However, it is unknown whether INMT is necessary for the biosynthesis of endogenous DMT. To test this, we generated a novel INMTknockout rat model and studied tryptamine methylatio... More

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