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STING induces LC3B lipidation onto single-membrane vesicles via the V-ATPase and ATG16L1-WD40 domain

J Cell Biol. 2020-12; 
Tara D Fischer, Chunxin Wang, Benjamin S Padman, Michael Lazarou, Richard J Youle
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Proteins, Expression, Isolation and Analysis … 10–20 μg of cell lysate per sample was loaded into 4–12% Bis-Tris gels (GenScript) and separated in MES or MOPs buffer (GenScript). Separated proteins were then transferred onto … Get A Quote

摘要

Following the detection of cytosolic double-stranded DNA from viral or bacterial infection in mammalian cells, cyclic dinucleotide activation of STING induces interferon β expression to initiate innate immune defenses. STING activation also induces LC3B lipidation, a classical but equivocal marker of autophagy, that promotes a cell-autonomous antiviral response that arose before evolution of the interferon pathway. We report that STING activation induces LC3B lipidation onto single-membrane perinuclear vesicles mediated by ATG16L1 via its WD40 domain, bypassing the requirement of canonical upstream autophagy machinery. This process is blocked by bafilomycin A1 that binds and inhibits the vacuolar ATPase (V-ATP... More

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