Glucose-Induced Enhanced Virulence in Strains of Multidrug-Resistant Pseudomonas aeruginosa Isolated from Diabetic Patients

Pseudomonas aeruginosa is known for its metabolic versatility and uses a variety of substrates; interestingly, glucose is not the favored carbon source. Although glucose is not readily utilized by them, there is a possibility that the increased susceptibility of diabetics to infections with P. aeruginosa is related to the effect of glucose on the expression of virulence genes. The curiosity in understanding the effect of glucose on virulence gene expression in P. aeruginosa and the lacuna of studies in this field prompted us to undertake the current investigation. It included the quantification of various virulence factors and their gene expression upon supplementation with glucose in clinical MDR P. aeruginosa isolates recovered from diabetics. Interestingly, the study observed a remarkable difference in the virulence attributes in the isolates with and without glucose supplementation. External glucose was found to be modulating the QS gene expression, thus altering the elaboration of other virulence factors. Variations in the gene expressions induced by glucose partly explain the increased susceptibility of diabetic patients to P. aeruginosa infections.