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Efficient production of isomaltulose using engineered Yarrowia lipolytica strain facilitated by non-yeast signal peptide-mediated cell surface display

J Sci Food Agric. 2024-03; 
Juanjuan Liu, Jiao Li, Peng Chen, Yan Zeng, Jiangang Yang, Yuanxia Sun
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Codon Optimization The encoding genes of sucrose isomerase PdSI from P. dispersa UQ68J (NCBI accession number: AY223549.1) and SpSI from S. plymuthica AS9 (NCBI accession number: KT734769.1) were codon-optimized according to usage bias in Y. lipolytica, synthesized in pET-28a by GenScript (Nanjing, China). Get A Quote

摘要

Background: Isomaltulose is a 'generally recognized as safe' ingredient and is widely used in the food, pharmaceutical and chemical industries. The exploration and development of efficient technologies is essential for enhancing isomaltulose yield. Results: In the present study, a simple and efficient surface display platform mediated by a non-yeast signal peptide was developed in Yarrowia lipolytica and utilized to efficiently produce isomaltulose from sucrose. We discovered that the signal peptide SP1 of sucrose isomerase from Pantoea dispersa UQ68J (PdSI) could guide SIs anchoring to the cell surface of Y. lipolytica, demonstrating a novel and simple cell surface display strategy. Furthermore, the PdSI ex... More

关键词

Yarrowia lipolytica; cell surface display; isomaltulose; signal peptide; sucrose isomerase; whole-cell biocatalysis.