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CRISPR/CasΦ2-mediated gene editing in wheat and rye

J Integr Plant Biol. 2024-04; 
Sanzeng Zhao , Xueying Han , Yachen Zhu , Yuwei Han , Huiyun Liu , Zhen Chen , Huifang Li , Dan Wang , Chaofan Tian , Yuting Yuan , Yajie Guo , Xiaomin Si , Daowen Wang, Xiang Ji
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Synthetic sgRNA and crRNA Service To prepare the constructs used in this study, TaU3::tRNA-crRNA was synthesized commercially (GenScript, Nanjing, China) for developing the crRNAs of different genes. Get A Quote

摘要

The compact CRISPR/CasΦ2 system provides a complementary genome engineering tool for efficient gene editing including cytosine and adenosine base editing in wheat and rye with high specificity, efficient use of the protospacer-adjacent motif TTN, and an alternative base-editing window.

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