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Covalent bicyclization of protein complexes yields durable quaternary structures

Chem. 2024-02; 
George H Hutchins, Sebastian Kiehstaller, Pascal Poc, Abigail H Lewis, Jisun Oh, Raya Sadighi, Nicholas M Pearce, Mohamed Ibrahim, Ivana Drienovská, Anouk M Rijs, Saskia Neubacher, Sven Hennig, Tom N Grossmann
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Proteins, Expression, Isolation and Analysis … homotrimeric protein complexes, thereby increasing protein … agent to form a protein assembly with bicyclic topology. … Samples were analysed by a 4–20% gradient gel (GenScript Get A Quote

摘要

Proteins are essential biomolecules and central to biotechnological applications. In many cases, assembly into higher-order structures is a prerequisite for protein function. Under conditions relevant for applications, protein integrity is often challenged, resulting in disassembly, aggregation, and loss of function. The stabilization of quaternary structure has proven challenging, particularly for trimeric and higher-order complexes, given the complexity of involved inter- and intramolecular interaction networks. Here, we describe the chemical bicyclization of homotrimeric protein complexes, thereby increasing protein resistance toward thermal and chemical stress. This approach involves the structure-based sel... More

关键词

Aggregation, INCYPRO, biocatalysis, bioconjugation, chemical biology, cross-linking, crystallography, enzymes, protein engineering, structural stabilization