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Codon Optimization> | … , YP 009724390.1) was obtained from the Wuhan-Hu-1 SARS-CoV-2 isolate (NCBI reference sequence: NC_045512.2) and was codon-optimized for rice and synthesized by GenScript … | Get A Quote |
The spike protein receptor-binding domain (RBD) of SARS-CoV-2 is required for the infection of human cells. It is the main target that elicits neutralizing antibodies and also a major component of diagnostic kits. The large demand for this protein has led to the use of plants as a production platform. However, it is necessary to determine the -glycan structures of an RBD to investigate its efficacy and functionality as a vaccine candidate or diagnostic reagent. Here, we analyzed the -glycan profile of the RBD produced in rice callus. Of the two potential -glycan acceptor sites, we found that one was not utilized and the other contained a mixture of complex-type -glycans. This differs from the heterogeneous mixt... More