至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The SARS-CoV-2 Spike Protein Receptor-Binding Domain Expressed in Rice Callus Features a Homogeneous Mix of Complex-Type Glycans

Int J Mol Sci. 2024-04; 
Guillermo Sobrino-Mengual, Victoria Armario-Nájera, Juliette Balieu, Marie-Laure Walet-Balieu, Andrea Saba-Mayoral, Ana M Pelacho, Teresa Capell, Paul Christou, Muriel Bardor, Patrice Lerouge
Products/Services Used Details Operation
Codon Optimization … , YP 009724390.1) was obtained from the Wuhan-Hu-1 SARS-CoV-2 isolate (NCBI reference sequence: NC_045512.2) and was codon-optimized for rice and synthesized by GenScript Get A Quote

摘要

The spike protein receptor-binding domain (RBD) of SARS-CoV-2 is required for the infection of human cells. It is the main target that elicits neutralizing antibodies and also a major component of diagnostic kits. The large demand for this protein has led to the use of plants as a production platform. However, it is necessary to determine the -glycan structures of an RBD to investigate its efficacy and functionality as a vaccine candidate or diagnostic reagent. Here, we analyzed the -glycan profile of the RBD produced in rice callus. Of the two potential -glycan acceptor sites, we found that one was not utilized and the other contained a mixture of complex-type -glycans. This differs from the heterogeneous mixt... More

关键词

SARS-CoV-2, biologics, glycan profile, plant molecular farming, receptor-binding domain, spike protein