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Ancient nitrogenases are ATP dependent

MBio. 2024-06; 
Derek F Harris, Holly R Rucker, Amanda K Garcia, Zhi-Yong Yang, Scott D Chang, Hannah Feinsilber, Betül Kaçar, Lance C Seefeldt
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摘要

Life depends on a conserved set of chemical energy currencies that are relics of early biochemistry. One of these is ATP, a molecule that, when paired with a divalent metal ion such as Mg, can be hydrolyzed to support numerous cellular and molecular processes. Despite its centrality to extant biochemistry, it is unclear whether ATP supported the function of ancient enzymes. We investigate the evolutionary necessity of ATP by experimentally reconstructing an ancestral variant of the N-reducing enzyme nitrogenase. The Proterozoic ancestor is predicted to be ~540-2,300 million years old, post-dating the Great Oxidation Event. Growth rates under nitrogen-fixing conditions are ~80% of those of wild type in . In the ... More

关键词

ancestral sequence reconstruction, energy, nitrogen fixation