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M tuberculosis PrrA binds the dosR promoter and regulates mycobacterial adaptation to hypoxia

Tuberculosis (Edinb). 2024-06; 
Yannik A Haller, Jiapei Jiang, Zijian Wan, Alexia Childress, Shaopeng Wang, Shelley E Haydel
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Custom Vector Construction … Tanya Parish; Addgene plasmid # 24659) were constructed by GenScript (GenScript, USA), substituting upstream P smyc for prrA Mtb , dosR Mtb , or cydA Mtb promoter sequences … Get A Quote

摘要

The PrrAB two-component system (TCS) is essential for Mycobacterium tuberculosis viability. Previously, it was demonstrated that PrrA binds DNA in the absence of PrrB-mediated transphosphorylation and that non-cognate serine/threonine-kinases phosphorylate PrrA threonine-6 (T6). Therefore, we investigated the differential binding affinity and regulatory properties of the M. tuberculosis-derived wild-type PrrA, PrrA phosphomimetic (D58E, T6E), and PrrA phosphoablative (D58A, T6A) proteins with the prrA, dosR, and cydA genes. While we hypothesized greater DNA binding affinity and more pronounced regulation by PrrA phosphomimetic variants, recombinant, wild-type PrrA bound DNA with greatest affinity. Collectively,... More

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