Research on adipogenesis will help to improve the meat quality of livestock. Long noncoding RNAs (lncRNAs) are involved in mammalian adipogenesis as epigenetic modulators. In this study, we analyzed lncRNA expression during bovine adipogenesis and detected 195 differentially expressed lncRNAs, including lncRNA , which was significantly upregulated in mature bovine adipocytes. Gain- and loss-of-function experiments confirmed that promoted the proliferation, apoptosis, and differentiation of bovine preadipocytes. RNA pull-down revealed that the nonmuscle myosin 10 (MYH10) is a potential binding protein of . Then, we elucidated that loss of caused increased ubiquitination of MYH10, which confirmed that regulate... More
Research on adipogenesis will help to improve the meat quality of livestock. Long noncoding RNAs (lncRNAs) are involved in mammalian adipogenesis as epigenetic modulators. In this study, we analyzed lncRNA expression during bovine adipogenesis and detected 195 differentially expressed lncRNAs, including lncRNA , which was significantly upregulated in mature bovine adipocytes. Gain- and loss-of-function experiments confirmed that promoted the proliferation, apoptosis, and differentiation of bovine preadipocytes. RNA pull-down revealed that the nonmuscle myosin 10 (MYH10) is a potential binding protein of . Then, we elucidated that loss of caused increased ubiquitination of MYH10, which confirmed that regulates adipogenesis by enhancing the stability of the MYH10 protein. Western blotting was used to demonstrate that activated the PI3K/Akt signaling pathway. Bioinformatic analysis and dual-luciferase reporter assays indicated that competitively absorbed . The overexpression and interference of in bovine preadipocytes displayed that inhibited proliferation, apoptosis, and differentiation. Further results suggested that targeted the gene and that controlled the proliferation of bovine preadipocytes by modulating the / axis. This study revealed the complex mechanisms of underlying bovine adipogenesis for the first time, which would provide useful information for genetics and breeding improvement of Chinese beef cattle.
