| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … After the successful sequencing verification of the pET-28a plasmid, the target gene was sent to GenScript for codon optimization, and the optimized target fragment was integrated into … | Get A Quote |
The 1092 bp F3H gene from Roxb., which was named TbF3H, was cloned and it encodes 363 amino acids. Bioinformatic and phylogenetic tree analyses revealed the high homology of TbF3H with flavanone 3-hydroxylase from other plants. A functional analysis showed that TbF3H of Roxb. encoded a functional flavanone 3-hydroxylase; it catalyzed the formation of dihydrokaempferol (DHK) from naringenin in . The promoter strengths were compared by fluorescence microscopy and flow cytometry detection of the fluorescence intensity of the reporter genes initiated by each constitutive promoter (FITC), and DHK production reached 216.7 mg/L by the promoter adjustment strategy and the optimization of fermentation conditions. The ... More
