| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … antibody anti-Adeno-associated virus (AAV), VP1/VP2/VP3, clone B1 (American Research Products, MA) was used at 1:1000 dilution, while primary antibody anti-His-tag (GenScript, NJ… | Get A Quote |
Adeno-associated Virus (AAV) is a promising vector for gene therapy. However, few studies have focused on producing virus-like particles (VLPs) of AAV in cells, especially in E. coli. In this study, we describe a method to produce empty VP3-only VLPs of AAV2 in E. coli by co-expressing VP3 and assembly-activating protein (AAP) of AAV2. Although the yields of VLPs produced with our method were low, the VLPs were able to self-assemble in E. coli without the need of in vitro capsid assembly. The produced VLPs were characterized by immunological detection and transmission electron microscopy (TEM). In conclusion, this study demonstrated that capsid assembly of AAV2 is possible in E. coli, and E. coli may be a candi... More
