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Cascaded amplifying circuit enables sensitive detection of fungal pathogens

Biosens Bioelectron. 2024-01; 
Cong Fan, Nike He, Jifeng Yuan
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Gene Synthesis … WHWVRLRKGQGLF, synthesized by GenScript), experiments … can eventually activate the reporter gene under the control of … Sc-G1.0 with EGFP gene under GAL1 promoter and strain Sc… Get A Quote

摘要

The rapid and accurate detection of fungal pathogens is of utmost importance in the fields of healthcare, food safety, and environmental monitoring. In this study, we implemented a cascaded amplifying circuit in Saccharomyces cerevisiae to improve the G protein-coupled receptor (GPCR) mediated fungal detection. The GPCR signaling pathway was coupled with the galactose-regulated (GAL) system and a positive feedback loop was implemented to enhance the performance of yeast biosensor. We systematically compared four generations of biosensors for detecting the mating pheromone of Candida albicans, and the best biosensor exhibited the limit of detection (LOD) as low as 0.25 pM and the limit of quantification (LOQ) of... More

关键词

Cascaded amplification, Fungal detection, GPCR, Synthetic biology, Yeast biosensor