| Products/Services Used | Details | Operation |
|---|---|---|
| Molecular Biology Reagents | … The M7D12H WT synthetic DNA sequence was ordered from GenScript Biotech (Rijswijk The Netherlands) and cloned into a pET22b (+) expression vector between NcoI and HindIII. … | Get A Quote |
Iontophoresis enables the non-invasive transdermal delivery of moderately-sized proteins and the needle-free cutaneous delivery of antibodies. However, simple descriptors of protein characteristics cannot accurately predict the feasibility of iontophoretic transport. This study investigated the cathodal and anodal iontophoretic transport of the negatively charged M7D12H nanobody and a series of negatively charged variants with single amino acid substitutions. Surprisingly, M7D12H and its variants were only delivered transdermally by anodal iontophoresis. In contrast, transdermal permeation after cathodal iontophoresis and passive diffusion was
