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A metal anion strategy to induce pyroptosis combined with STING activation to synergistically amplify anti-tumor immunity

Materials Today . 2024-08; 
Zifan Pei , Nan Jiang , Fei Gong , Weihao Yang , Jiachen Xu , Bin Yu , Nailin Yang , Jie Wu , Huali Lei , Shumin Sun , Longxiao Li , Zhicheng Liu , Caifang Ni , Liang Cheng
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Proteins, Expression, Isolation and Analysis After adding equal amounts of protein and SDS-PAGE separation (GenScript, S-7 Catalog: M00928), the proteins were transferred to polyvinylidene difluoride (PVDF) membranes and then blocked with 5% bovine serum albumin (BSA) for 1 h. Get A Quote

摘要

Growing evidence has demonstrated the positive role of bioactive metal ions in enhancing pyroptosis-mediated cancer immunotherapy. However, further amplification of the sustained immune response remains challenging. Herein, by selecting from typical metal anions, we confirmed the significant cytotoxicity and pyroptosis induction potency of vanadate anions, owing to the inhibition of ATPases and disruption of intracellular ion homeostasis. Then, PEGylated bimetallic manganese vanadate nanoparticles (MnVOx) were synthesized for stimulator of interferon genes (STING) pathway-boosted pyroptosis therapy. The vanadate produced from MnVOx degradation inhibited membrane ATPases and induced potassium efflux and calcium ... More

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