Products/Services Used | Details | Operation |
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Catalog Peptides> | G4 helicase assay was performed as previously described28,71 with minor modifications. Both intermolecular (OX-1 G4) and intramolecular (c-myc G4) G4 DNA were prepared as previously described28,72 and labelled with FAM (GenScript). | Get A Quote |
Triplex DNA structures, formed when a third DNA strand wraps around the major groove of DNA, are key molecular regulators and genomic threats. However, the regulatory network governing triplex DNA dynamics remains poorly understood. Here we reveal the binding and functional repertoire of proteins that interact with triplex DNA through chemoproteomic profiling in living cells. We develop a chemical probe that exhibits exceptional specificity towards triplex DNA. By employing a co-binding-mediated proximity capture strategy, we enrich triplex DNA interactome for quantitative proteomics analysis. This enables the identification of a comprehensive list of proteins that interact with triplex DNA, characterized by di... More