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Tuning the fluidity and protein corona of ultrasound-responsive liposomal nanovaccines to program T cell immunity in mice

Nature Communications. 2024-09; 
Jia He , Chaoyu Wang , Xiao Fang , Junyao Li , Xueying Shen , Junxia Zhang , Cheng Peng , Hongjian Li , Sai Li , Jeffrey M Karp , Rui Kuai
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Click Peptide Services Briefly, CSSSIINFEKL, CRAHYNIVTF, or CTAPDNLGYM (Genscript) was reacted with Dioleoyl-sn-glycero-3-phosphoethanolamine-N-[3-(2-pyridyldithio) propionate] (Sigma) at a 1.5:1 molar ratio in DMF for 3 h at room temperature. The obtained phospholipid-antigen was diluted 10X with DI water and freeze-dried before preparing liposomal nanovaccines. Get A Quote

摘要

Inducing high levels of antigen-specific CD8α+ T cells in the tumor is beneficial for cancer immunotherapy, but achieving this in a safe and effective manner remains challenging. Here, we have developed a designer liposomal nanovaccine containing a sonosensitizer (LNVS) to efficiently program T cell immunity in mice. Following intravenous injection, LNVS accumulates in the spleen in a protein corona and fluidity-dependent manner, leading to greater frequencies of antigen-specific CD8α+ T cells than soluble vaccines (the mixture of antigens and adjuvants). Meanwhile, some LNVS passively accumulates in the tumor, where it responds to ultrasound (US) to increase the levels of chemokines and adhesion molecules th... More

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