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Lipid synthesis, triggered by PPARγ T166 dephosphorylation, sustains reparative function of macrophages during tissue repair

Nature Communications. 2024-08; 
Shiman Zuo , Yuxin Wang , Hanjing Bao , Zehui Zhang , Nanfei Yang , Meng Jia , Qing Zhang , Ani Jian , Rong Ji , Lidan Zhang , Yan Lu , Yahong Huang , Pingping Shen
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Gene Synthesis Mice were anaesthetized with isoflurane and 50 μl of 12 μM cardiotoxin (CTX) (RP17303, GenScript) was injected in the tibialis ante-rior muscle. Get A Quote

摘要

Macrophages may acquire a reparative phenotype that supports tissue repair and remodeling in response to tissue injury. However, the metabolic requirements underpinning this process are incompletely understood. Here, we show that posttranslational modification (PTM) of PPARγ regulates lipid synthesis in response to wound microenvironmental cues and that metabolic rewiring orchestrates function of reparative macrophages. In injured tissues, repair signaling leads to decreased macrophage PPARγ threonine 166 (T166) phosphorylation, which results in a partially active PPARγ transcriptional program comprised of increased binding activity to the regulator regions of lipid synthesis-associated genes, thereby increa... More

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