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Natural SNP Variation in GbOSM1 Promotor Enhances Verticillium Wilt Resistance in Cotton

Advanced Science. 2024-10; 
Guilin Wang, Dayong Zhang , Haitang Wang , Jinmin Kong , Zhiguo Chen , Chaofeng Ruan , Chaoyang Deng , Qihang Zheng , Zhan Guo , Hanqiao Liu, Weixi Li , Xinyu Wang , Wangzhen Guo
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Proteins, Expression, Isolation and Analysis Ni-NTA Agarose Resin (L00250, Genscript, Nanjing, China) the HisGbOSM1 protein was detected by immunoblotting using an anti-His antibody (A02003, Genscript, Nanjing, China) Get A Quote

摘要

Osmotin is classified as the pathogenesis-related protein 5 group. However, its molecular mechanism involved in plant disease resistance remains largely unknown. Here, a Verticillium wilt (VW) resistance-related osmotin gene is identified in Gossypium barbadense (Gb), GbOSM1. GbOSM1 is preferentially expressed in the roots of disease-resistant G. barbadense acc. Hai7124 and highly induced by Verticillium dahliae (Vd). Silencing GbOSM1 reduces the VW resistance of Hai7124, while overexpression of GbOSM1 in disease-susceptible G. hirsutum improves tolerance. GbOSM1 predominantly localizes in tonoplasts, while it relocates to the apoplast upon exposure to osmotic stress or Vd infection. GbOSM1 confers VW resistanc... More

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