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A Tag-Free Platform for Synthesis and Screening of Cyclic Peptide Libraries

Angew Chem Int Ed Engl . 2024-05; 
Angele Bruce , Victor Adebomi , Patrick Czabala , Jonathan Palmer , William M McFadden , Zachary C Lorson , Ryan L Slack , Gaurav Bhardwaj , Stefan G Sarafianos , Monika Raj
Products/Services Used Details Operation
PCR Cloning and Subcloning Subsequently, a 6xHIS tag was added to the Cterminal end of CAHEX to create the CAHEX:6HIS construct in pET11a vector (Genscript). Get A Quote

摘要

In the realm of high-throughput screening (HTS), macrocyclic peptide libraries traditionally necessitate decoding tags, essential for both library synthesis and identifying hit peptide sequences post-screening. Our innovation introduces a tag-free technology platform for synthesizing cyclic peptide libraries in solution and facilitates screening against biological targets to identify peptide binders through unconventional intramolecular CyClick and DeClick chemistries (CCDC) discovered through our research. This combination allows for the synthesis of diverse cyclic peptide libraries, the incorporation of various amino acids, and facile linearization and decoding of cyclic peptide binder sequences. Our sensitiv... More

关键词

Affinity Selection; CyClick Chemistry; Cyclic Peptide Libraries; Peptide Macrocyclization; Tag-Free Approaches.