| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | CTCTTTGATGTCAC-3′. Western Blot Analysis. Proteins from the bladder were extracted and quantified according to the manufacturer’s protocol (GenScript, Nanjing, China). Clarity Western ECL Substrate (BIO-RAD, California, USA) was used to visualize the protein bands and was captured by using an ePhoto (GenScript, Nanjing, China). | Get A Quote |
Neuromuscular conduction dysfunction-induced underactive bladder (UAB) is a major urological complication associated with diabetes mellitus (DM), and there remain deficiencies in reliable pharmacological treatment options. Astragalus polysaccharides (APS), as an edible active substance in Astragalus membranaceus, have a therapeutic effect on diabetes and its complications. We investigated the effects and mechanism of APS in high-fat-diet-induced (HFD) diabetic UAB mice. APS significantly reduced fasting plasma glucose, insulin, and HOMA-IR index. Furthermore, APS treatment significantly decreased maximum bladder capacity, residual volume, bladder compliance, contraction intervals, empty and full resting pressur... More
