GenRCA Rare Codon Analysis Tool provides comprehensive evaluation of codon usage preferences for coding sequence(s) of interest, supporting up to 31 codon preference indices and 65 expression host organisms. The provided analysis report could serve as an informative reference to evaluate whether a further codon optimization is required for heterologous expression. The list of included codon usage bias indices can be downloaded here.
If you find our tool useful, please cite our paper using the following:
Fan, K., Li, Y., Chen, Z. et al. GenRCA: a user-friendly rare codon analysis tool for comprehensive evaluation of codon usage preferences based on coding sequences in genomes. BMC Bioinformatics 25, 309 (2024). https://doi.org/10.1186/s12859-024-05934-z
Step 1: Select expression host organism along with its reference source.
Step 2: Enter coding sequence(s) into the table.
Step 3: Click "Analysis" button to view analysis result.
