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Catalog Products | ... A549 cells (seeded at 4.5 × 105 cells/ml in 60-mm culture dishes) were cultured overnight and treated with exendin-4 (AnaSpec, Fremont, CA), 5-azacyti- dine (Aza, Wako Pure Chem. Ind., Ltd., Osaka, Japan) or exendin- (9-39) (GenScript, Piscataway, NJ). ... | Get A Quote |
Exendin-4 is an agonist of the glucagon-like peptide 1 receptor (GLP-1R) and is used in the treatment of type 2 diabetes. Since human GLP-1R has been identified in various cells besides pancreatic cells, exendin-4 is expected to exert extrapancreatic actions. It has also been suggested to affect gene expression through epigenetic regulation, such as DNA methylation and/or histone modifications. Furthermore, the expression of extracellular-superoxide dismutase (EC-SOD), a major SOD isozyme that is crucially involved in redox homeostasis, is regulated by epigenetic factors. In the present study, we demonstrated that exendin-4 induced the demethylation of DNA in A549 cells, which, in turn, affected the expression ... More