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Molecular Biology Tools> | ... (2009), was performed accord- ing to Pieta et al. (2014), and relative gene expression was determined using RT-qPCR. Primers were using the GenScript tool (https://www.genscript.com/ tools/real-time- pcr-tagman-primer-design-tool) based on the genes that were ... | Get A Quote |
Listeria monocytogenes is a pathogenic microorganism in humans and is frequently transmitted by food. Methods to control the presence of Listeria in foods are necessary. In the present study, transcriptomics of L. monocytogenes grown in the presence of essential oil extracted from Baccharis psiadioides were studied by RNA sequencing and reverse transcription quantitative polymerase chain reaction (RT-qPCR) experiments. The results obtained indicate that essential oil of B. psiadioides has potential bacteriostatic activity at the concentration tested, affecting Listeria cells functioning and development. Responses of the microorganism included upregulation of stress genes and downregulation of virulence genes, s... More