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Catalog Products> | ... GST bind resin was used in this assay, and the pull-down was carried out as described previously [13]. Approximately 200 μg of purified GST-PcL-lectin was incubated with GST bind resin (GenScript, Nanjing, China) for 1 h, washed with PBS. … | Get A Quote |
L-type lectins are involved in glycoproteins secretory pathways and are associated with many immune responses. There is growing evidence that L-type lectins are also involved in viral replication. In this study, a novel L-type lectin (named as PcL-lectin) was identified from red swamp crayfish (Procambarus clakii). Gene sequencing and phylogenetic tree analysis results showed that the PcL-lectin was a kind of endoplasmic reticulum Golgi intermediate compartment-53 (ERGIC-53). The expression level of PcL-lectin was significantly down regulated in crayfish after challenged with white spot syndrome virus (WSSV). Recombinant PcL-lectin protein facilitated the replication of WSSV in crayfish. In addition, WSSV repli... More