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The involvement of protein kinase G inhibitor in regulation of apoptosis and autophagy markers in spatial memory deficit induced by Aβ.

Fundam Clin Pharmacol.. 2016-08; 
Shariatpanahi M, Khodagholi F, Ashabi G, Bonakdar Yazdi B, Hassani S, Azami K, Abdollahi M, Noorbakhsh F, Taghizadeh G, Sharifzadeh M.
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Catalog Products ... Drug infusion was carried out in about one minute. Drugs preparations and infusions Aβ (1-42) (Genscript, Piscataway, NJ, USA) was dissolved in PBS and was incubated for 5 days at 37 ◦C. Final concentration of 30 (ng/μl) of Aβ was prepared for injection. KT5823 (Sigma, St. . Get A Quote

摘要

The role of nitric oxide/protein kinase G (NO/PKG) in neurodegenerative disorders is controversial in different circumstances. PKG affects neurons both by itself and as a result of increased NO concentration. In this study, we examined the influence of PKG on spatial memory by intrahippocampal administration of three different concentrations of KT5823 as a PKG inhibitor. Morris water maze (MWM) was used for evaluation of behavioral alterations. We also measured the apoptosis and autophagy markers as two probable interfering pathways with PKG signaling by Western blot method. We found that in Aβ-pretreated rats, intrahippocampus infusions of 2.5, 5, and 10 μm/side of KT5823 led to a significant reduction in es... More

关键词

KT5823; Morris water maze; amyloid beta; autophagy; caspase-3; protein kinase G