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Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium.

J Control Release.. 2017-09; 
de Groot AM, Du G, Mönkäre J, Platteel ACM, Broere F, Bouwstra JA, Sijts AJAM.
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Catalog Products ... In short, 2.5 × 10 6 erythrocyte-depleted splenocytes were incubated in complete RPMI medium with 1 μg/ml pOVA 257–264 (Genscript) or complete RPMI medium and 10 Μm monensin (eBioscience) for 6 h at 37 °C in 6% humidified incubator. ... Get A Quote

摘要

The skin is an attractive organ for immunization due to the presence of a large number of epidermal and dermal antigen-presenting cells. Hollow microneedles allow for precise and non-invasive intradermal delivery of vaccines. In this study, ovalbumin (OVA)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles with and without TLR3 agonist poly(I:C) were prepared and administered intradermally by hollow microneedles. The capacity of the PLGA nanoparticles to induce a cytotoxic T cell response, contributing to protection against intracellular pathogens, was examined. We show that a single injection of OVA-loaded PLGA nanoparticles, compared to soluble OVA, primed both adoptively transferred antigen-specific n... More

关键词

Cytotoxic T cell response; Hollow microneedles; Intradermal immunization; PLGA nanoparticles; Protein vaccine