| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | ... 25 mM Tris (pH 7.9), 150 mM NaCl, and 1 mM dithiothreitol (DTT). The supernatant was incubated with glutathione Sepharose beads (Genscript, Nanjing, China). After rinsing with wash buffer (40 mM Tris pH 7.9, 500 mM NaCl ... | Get A Quote |
Scanning tunneling microscopy (STM) can be used to image individual biological molecules, such as proteins, in vacuum or air. This requires sample dehydration and thus may not reflect the native state of the molecule. Extensive efforts have been made to image single proteins in solution using STM; however, the images have revealed only round or oval shapes with no sub-molecular details. Here, we present the sub-molecular features of streptavidin proteins under physiological conditions using a homebuilt low-leakage-current and highstability liquid phase STM. The N-lobe, C-lobe, and C-terminal tail of the epidermal growth factor receptor kinase domains were also resolved in solution. Our results demonstrate that ... More
