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Dimensionality and spreading influence MSC YAP/TAZ signaling in hydrogel environments.

Biomaterials.. 2016-10; 
Caliari SR, Vega SL, Kwon M, Soulas EM, Burdick JA.
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Catalog Products ... 4 wt% NorHA hydrogels were photopolymerized (2 mW cm -2 ) in the presence of 2 mM lithium acylphosphinate (LAP) [22] and [23] initiator for either 2 min (2D) or 5 min (3D) in the presence of thiolated RGD (GCGYGRGDSPG, GenScript) and either the non-degradable or ... Get A Quote

摘要

Improved fundamental understanding of how cells interpret microenvironmental signals is integral to designing better biomaterial therapies. YAP/TAZ are key mediators of mechanosensitive signaling; however, it is not clear how they are regulated by the complex interplay of microenvironmental factors (e.g., stiffness and degradability) and culture dimensionality. Using covalently crosslinked norbornene-functionalized hyaluronic acid (HA) hydrogels with controlled stiffness (via crosslink density) and degradability (via susceptibility of crosslinks to proteolysis), we found that human mesenchymal stem cells (MSCs) displayed increased spreading and YAP/TAZ nuclear localization when cultured atop stiffer hydrogels; ... More

关键词

Degradation; Dimensionality; Hydrogel; Mechanotransduction; Stiffness; YAP/TAZ