Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | ... Paddock et al., 2007). Codon usage was optimized for its expression in E. coli (GenScript). The open reading frame coding for the fragment M44-T108 was cloned between NdeI and XhoI of pET22b(+) vector. Using this construct ... | Get A Quote |
Bacterial Expression System> | Get A Quote |
MitoNEET is the first identified Fe-S protein anchored to mammalian outer mitochondrial membranes with the vast majority of the protein polypeptide located in the cytosol, including its [2Fe-2S] cluster-binding domain. The coordination of the cluster is unusual and involves three cysteines and one histidine. MitoNEET is capable of transferring its redox-active Fe-S cluster to a bacterial apo-ferredoxin in vitro even under aerobic conditions, unlike other Fe-S transfer proteins such as ISCU. This specificity suggests its possible involvement in Fe-S repair after oxidative and/or nitrosative stress. Recently, we identified cytosolic aconitase/iron regulatory protein 1 (IRP1) as the first physiological protein acc... More