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Disruption of the E. coli LptC dimerization interface and characterization of lipopolysaccharide and LptA binding to monomeric LptC

Protein Sci.. 2018-08; 
Schultz KM, Fischer MA, Noey EL, Klug CS.
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Mutagenesis Services ...Mutations were introduced into the gene of the LptC soluble domain (amino acids 24–191) using High Fidelity PCR EcoDry Premix (Clontech, Mountain View, CA) and sequencing at Retrogen (San Diego, CA) or by commercial gene synthesis or sitedirected mutagenesis (<b>GenScript</b>, Piscataway, NJ)... Get A Quote
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摘要

Lipopolysaccharide (LPS) is an essential element of nearly all Gram-negative bacterial outer membranes and serves to protect the cell from adverse environmental stresses. Seven members of the lipopolysaccharide transport (Lpt) protein family function together to transport LPS from the inner membrane (IM) to the outer leaflet of the outer membrane of bacteria such as Escherichia coli. Each of these proteins has a solved crystal structure, including LptC, which is a largely periplasmic protein that is associated with the IM LptB2 FG complex and anchored to the membrane by an N-terminal helix. LptC directly binds LPS and is hypothesized to be involved in the transfer of LPS to another periplasmic protein, LptA. Pu... More

关键词

EPR spectroscopy; LPS; LPS binding protein; LptC; computational modeling; dimer; dissociation constant; electrostatic surface potential; lipopolysaccharide; periplasmic protein