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The C-terminal tail of the NEIL1 DNA glycosylase interacts with the human mitochondrial single-stranded DNA binding protein.

DNA Repair (Amst.). 2018-01; 
SharmaNidhi,ChakravarthySrinivas,LongleyMatthew J,CopelandWilliam C,PrakashAishw
Products/Services Used Details Operation
Gene Synthesis … were described previously [32,39]. A C-terminal GST fused NEIL1 construct comprising residues 289–389 was synthesized by Genscript (Piscataway, NJ) and subcloned into the pGEX-4T2 vector. The pGEX-4T2 GST fused … Get A Quote
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摘要

The 16.5 kb mitochondrial genome is subjected to damage from reactive oxygen species (ROS) generated in the cell during normal cellular metabolism and external sources such as ionizing radiation and ultraviolet light. ROS cause harmful damage to DNA bases that could result in mutagenesis and various diseases, if not properly repaired. The base excision repair (BER) pathway is the primary pathway involved in maintaining the integrity of mtDNA. Several enzymes that partake in BER within the nucleus have also been identified in the mitochondria. The nei-like (NEIL) DNA glycosylases initiate BER by excising oxidized pyrimidine bases and others such as the ring-opened formamidopyrimidine and the hydantoin lesion... More

关键词

Base excision repair,Mitochondrial single-stranded DNA binding protein,NEIL1 DNA glycosylase,Protein painting,Size exclusion chromatography,Small angle X-ray scatte