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Search for independent (β/α)4 subdomains in a (β/α)8 barrel β-glucosidase.

PLoS ONE. 2018; 
AlmeidaVitor M,FrutuosoMaira A,MaranaSand
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ORF cDNA Clones/MolecularCloud … bglTm proteins. The DNA segments encoding the wild-type and mutant bglTm proteins were produced by GenScript (Piscataway, NJ, US) and cloned into the pLATE51 expression vector (Thermo Scientific, Waltham, MA, US) … Get A Quote

摘要

Proteins that fold as (β/α)8 barrels are thought to have evolved from half-barrels that underwent duplication and fusion events. The evidence is particularly clear for small barrels, which have almost identical halves. Additionally, computational calculations of the thermodynamic stability of these structures in the presence of denaturants have revealed that (β/α)8 barrels contain two subunits or domains corresponding to half-barrels. Hence, within (β/α)8 barrels, half-barrels are self-contained units. Here, we tested this hypothesis using β-glucosidase from the bacterium Thermotoga maritima (bglTm), which has a (β/α)8 barrel structure. Mutations were introduced to disrupt the noncovalent c... More

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