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Real-time monitoring of the Trojan-horse effect of silver nanoparticles by using a genetically encoded fluorescent cell sensor.

Nanoscale. 2018-04; 
YouFang,TangWenqin,YungLin-Yue L
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ORF cDNA Clones/MolecularCloud … Generation of the MT2a-FRET protein sensor. cDNA of MT2a was synthesized by GenScript® and was cloned into YC3.6/pRSETb and YC3.6/pcDNA3 plasmids using SphI and SacI sites in the plasmids to generate MT2a-FRET/pRSETb and MT2a-FRET/pcDNA3 … Get A Quote

摘要

Silver nanoparticles (AgNPs) are widely incorporated into commercial products due to their antimicrobial properties. As a consequence, concerns about the adverse effects induced by AgNPs to humans and the environment need to be carefully examined. The existing literature reveals that AgNPs exhibit certain toxic effects, but it remains to be proved whether AgNPs or the ionic silver (Ag+) released from AgNPs are the main toxic species. Here, a genetically encoded fluorescent protein sensor with high affinity to Ag+ was developed. The resulting sensor, MT2a-FRET, was found to be ratiometric, sensitive and selective toward only Ag+ but inert against AgNPs. This makes this sensor a potential useful tool ... More

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